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Br J Haematol 166

Cells were starved for nitrogen in a carbon free M9 medium for 3 h at 37 C, harvested by centrifugation and adjusted to the optical density of 3.0 in 20 mM TBS buffer (pH-7.2)

Additionally, many NRF2-regulated metabolic pathways were found to confer growth advantages to cancer cells, such as the upregulation of the PPP, nucleotide synthesis, and NADPH production

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